Andrej ŠUŠEK
pp. 37-45
ABSTRACT
Three naturally-growing local populations of Christmas rose (Helleborusniger L.) from two locations in Slovenia and one from neighbouring Croatia, were selected for molecular analysis based on AFLPs. 27 primer pairs were evaluated during the selective amplification step of the AFLP, and ten primer combinations were generated on the basis of the number of bands. The results from molecular analysis showed that the variation within populations was larger than that between populations. The molecular analysis showed that the genetic differences amongst populations were not high enough to state that the individuals of H. nigerwere grouped in clearly distinctive clusters. Small differences between the two Alpine populations (Bohinjska Bela and Peca) suggest that these are not isolated at the level of sexual reproduction. The migration of genes through pollen dissemination is probably always present, although pollen cannot migrate far.
Key words: Christmas rose, Helleborus niger, population diversity, molecular markers, AFLP
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