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Home Issues Issue 14 Micropropagation of roses (Rosa spp.): The effects of different media on in vitro rooting

Micropropagation of roses (Rosa spp.): The effects of different media on in vitro rooting

Metka ŠIŠKO

pp. 19-22

In vitro propagation techniques were studied in 3 different genotypes of roses (Rosa spp.) and the effects of two different disinfectants (dichloroisocyanuric acid and sodium hypochlorite) were evaluated. The sterilisation based ondichloroisocyanuric acid was more efficient (32.6% of vital explants) when compared to the sterilisation with sodium hypochlorite (7.3% of vital explants). The sterilised and vital explants were transferred onto a shoot proliferation medium based on MS. The subcultivation period lasted 4 months. From the first genotype, 186 shoots were obtained, from the second 219 and from the third only 88 shoots. Sufficiently developed plants were transferred to three different rooting media which differed in the presence of auxin (IBA), sucrose and MS concentration. The results show, that the rooting medium did not have the same effects on all tested genotypes. The first genotype resulted in 82% rooted plants on the medium with 1/2 MS supplemented with 30 g/L of sucrose and no IBA added. The most successful rooting for second genotype (55.6% rooted plants) was obtained on the rooting medium with full strength MS, supplemented with 30 g/L sucrose and 0.5 mg/L IBA. The third genotype rooted most successfully (65.9% plants with developed roots) on the medium with 1/4 MS supplemented with 30 g/L of sucrose and no IBA added. Rooted plants were transplanted into the substrate and acclimatised in the laboratory (common growth chamber) and in the greenhouse (moist chamber). The acclimatisation in the moist chamber had a positive effect on the number of survived plants (90.9% survival) compared with the acclimatisation in the common growth chamber (68.1% survival).

Key words: rose, Rosa spp. micropropagation, in vitro propagation, in vitro rooting 

Slovenian:
 
Mikropropagacija vrtnic (Rosa spp.): vpliv različnih gojišč na koreninjenje in vitro

Proučeni so bili vplivi različnih postopkov sterilizacije in koncentracije rastnih regulatorjev na vzpostavitev tkivne kulture, namnožitev in koreninjenje vrtnice in vitro. Tehnike razmnoževanja in vitro so bile proučevane pri treh različnih genotipih vrtnic. Od dveh uporabljenih metod sterilizacije, se je sterilizacija z dikloroizocianurno kislino izkazala kot bolj učinkovita (32,6 % vitalnih izsečkov) v primerjavi z natrijevim hipokloritom, kjer je bilo uspešno steriliziranih in vitalnih 7,3% izsečkov. Sterilni poganjki so bili premeščeni na gojišče za namnoževanje (MS) za 4 tedne. Pri prvem genotipu se je v tem obdobju namnožilo 186 poganjkov, na drugem 219 poganjkov in pri tretjem samo 88 poganjkov. Dobro razviti poganjki so bili preneseni na tri gojišča za koreninjenje, ki so se med seboj razlikovala po vsebnosti avksina (IBA), saharoze in MS. Rezultati so pokazali, da gojišča za koreninjenje niso enako vplivala na vse tri genotipe. Prvi genotip je najbolje koreninil (82%) na gojišču z ½ MS, 30mg/l saharoze in brez dodane IBA. Drugi genotip je najbolje koreninil (55,6%) na gojišču z originalnim MS, 30 g/l saharoze in 0,5 mg/l IBA. Tretji genotip je najbolje koreninil na gojišču z ¼ MS, 30 g/l saharoze in brez dodane IBA (65,9 % koreninjenih poganjkov). Koreninjeni poganjki so bili posajeni v substrat in aklimatizirani v laboratoriju oz. v meglilni komori rastlinjaka. Aklimatizacija v meglilni komori je bila bolj učinkovita (90,9 % aklimatiziranih sadik) v primerjavo s klasično aklimatizacijo, kjer je preživelo 68,1 % rastlin.

Ključne besede: vrtnica / mikropropagacija vrtnic / razmnoževanje in vitro / koreninjenje in vitro


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