Tina UGULIN1, Tamas BAKONYI, Rebeka BERČIČ, Andreja URBANEK KRAJNC

pp. 41-47

ABSTRACT

Preserving the plant genetic resources of genus Morus is insufficient but undoubtedly vital for conservation of the world’s germplasm for our successors. This research was focused on old mulberry varieties from the Gorizia region in Slovenia which were assessed for their contents on crucial metabolites (proteins, phenolics and thiols) in leaves regarding their antioxidant and nutraceutical potentials. Total proteins were measured spectrophotometrically by following the procedure of Bradford, the total phenolic contents were determined using the Folin-Ciocalteu method and thiols were established with monobromobimane fluorescent dye. The presented metabolite screening showed that some of the evaluated genotypes had higher concentrations of glutathione and were superior in contents of proteins and phenolics when compared to the results of other authors and could be propagated as highly recommendable feed for silkworms, and other animals.

Key words: Zea mays L. saccharata feeds, glutathione, Morus, phenols, proteins

Slovenian:

Spremenljivost vsebnosti skupnih proteinov, fenolov in tiolov, v listih starih genotipov murv v Goriški regiji

Ohranjanje genskih virov rodu Morus je nezadostno, čeprav je nedvomno bistvenega pomena za ohranjanje svetovne zapuščine za naše naslednike. Ta raziskava temelji na določitvi ključnih metabolitov (proteinov, fenolov in tiolov) v listih starih genotipovmurv iz Goriške regije v Sloveniji z namenom proučitve antioksidativne aktivnosti in hranilne vrednosti. Skupni proteini in fenoli so bili določeni spektrofotometrično s pomočjo Bradfordove in Folin-Ciocalteu metode, tioli so bili določeni s pomočjo HPLC po predhodnem markiranju s fluorescenčnim barvilom monobromobimane. Rezultati določitve metabolitov kažejo, da imajo nekateri genotipi visoko vsebnost glutationa,v primerjavi z rezultati drugih avtorjev so superiorni v vsebnosti proteinov in fenolov in bodov nadaljnjih raziskavah razmnoženi z namenom uporabe listov za krmo sviloprejk in ostalih živali.

Ključne besede: /

REFERENCES:

1. Ainsworth EA, Gillespie KM. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent. Nat. Protoc. 2007;2:875-877.

2. Ba NX, Giang VD, Ngoan LD. Ensiling of mulberry foliage (Morus alba) and the nutritive value of mulberry foliage silage for goats in central Vietnam. Livest. Res. Rural Develop. 2005;17(15).

3. Bakshi MPS, Wadhawa M. Tree leaves as complete feed for goat bucks. Small Rum. Res. 2006;69:74-78.

4. Bradford MM. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976;72:248-254.

5. Dixon DP, Cummins I, Cole DJ, Edwards R. Glutathione-mediated detoxification systems in plants. Curr. Opin. Plant Biology. 1998;1:258-266.

6. Flaczyk E, Kobus-Cisowska J, Przeor M, Korzak J, Remiszewski M, Korbas E, Buchowski M. Chemical characterization and antioxidative properties of Polish variety of Morus alba L. leaf aqueous extract from the laboratory and pilot-scale processes. Agricul. Sci. 2013;4:141-147.

7. Frottin F, Espagne C, Traverso JA, Mauve C, Valot B, Lelarge-Trouverie C, Zivy M, Noctor G, Meinnel T, Giglione C. Cotranslational proteolysis dominates glutathione homeostasis to support proper growth and development. Plant Cell 2009;21(10):3296-314.

8. Herbinger K, Then C, Löw M, Haberer K, Alexous M, Koch N, Remele K, Heerdt C, Grill D, Rennenberg H, Häberle K-H, Matyssek R, Tausz M, Weiser G. Tree age dependence and within-canopy variation of leaf gas exchange and antioxidative defence in Fagus sylvatica under experimental free-air ozone exposure. Environ. Pollut. 2015;137:476-482.

9. Ipavec VM. Murve in kavalirji. Svilogojstvo na Goriškem.Inštitut za slovensko narodopisje, Ljubljana, 2008.

10. Kandylis K, Hadjigeorgiu I, Harizanis P. The nutritive value of mulberry leaves (Morus alba) as a feed supplement for sheep. Trop. Anim. Health Pro. 2008;41:17-24.

11. Katsube T, Imawaka N, Kawano Y, Yamazaki Y, Shiwaku K, Yamane Y. Antioxidant flavonol glycosides in mulberry (Morus alba L.) leaves isolated based on LDL antioxidant activity. Food Chem. 2006;97:25-31.

12. Kim DS, Kang YM, Jin WM, Sung YY, Choi G, Kim HK. Antioxidant activities and polyphenol content of Morus alba leaf extracts collected from varying regions. Biomed. rep. 2014;2:675-680.

13. Kumar RV, Cahuhan S, Kumar D, More N. Nutritional composition in leaves of some mulberry varieties: A comparative study. Bioinformatics and biomedical technology, 2010 International conference. 2010;438-442.

14. Krishnaswami S, Noamani KR, Asan M. Studies on quality of mulberry leaves and silkworm cocoon crop production, Part I, Quality difference due to varieties. Indian J. Sericult. 1970;9(1):1-10.

15. Liu JX, Yao J, Yan B, Yu JQ, Shi ZQ. Effects of mulberry leaves to replace rapeseed meal on performance of sheep feeding on ammoniated rice straw diet. Small Rum. Res. 2001;39:131-136.

16. Ma ST, Liu DL, Deng JJ, Peng YJ. Protective effect of mulberry flavonoids on sciatic nerve in alloxan-induced diabetic rats. Braz. J Pharm. Sci. 2014;50(4):765-771.

17. Martinez M, Motta W, Cervera C, Pla M. Feeding mulberry leaves to fattening rabbits: effects of growth, carcass characteristics and meat quality. Animal Sci. 2005;80(3):275-280.

18. Matei A, Tanase DB, Diaconescu C, Constatinescu M, Dolis M. Contributions to the study of the leaf protein value in different mulberry varieties. Arch. Zootech. 2006;9.

19. Memon AA, Memon N, Luthria DL, Bhanger MI, Pitafi AA. Phenolic acids profiling and antioxidant potential of mulberry (Morus laevigata W., Morus nigra L., Morus alba L.) leaves and fruits grown in Pakistan. Pol. J. Food Nutr. Sci. 2010;60:25-32.

20. Nakamura M, Nakamura S, Oku T. Suppressive response of confections containing the extractive from leaves of Morus alba on postprandial blood glucose and insulin in healthy human subjects. Nutr. Metab. 2009;29.

21. Nepal M.P. Systematics and reproductive biology of the genus Morus L. (Moraceae) [dissertation]. Retrieved from ProQuest Dissertations and Theses, 2008 (Accession Order No.3325640.).

22. Noctor G, Mhamdi A, Chaouch S, Han Y, Neukermans J, Marquez-Garcia B, Queval G, Foyer CH. Glutathione in plants: an integrated overview. Plant Cell Environ. 2011;35:454-484.

23. Oh KS, Ryu SY, Lee S, Seo HW, Oh BK, Kim YS, Lee BH. Melanin-concentrating hormone-1 receptor antagonism and anti-obesity effects of ethanolic extract from Morus alba leaves in diet-induced obese mice. J.Ethnopharmacol. 2009;122:216-220.

24. Radojković MM, Zeković ZP, Vidovič SS, Kočar DD, Mašković PZ. Free radical scavenging activity and total phenolic and flavonoid contents of mulberry (Morus spp. L., Moraceae) extracts. Hem. Ind. 2012;66:547-552.

25. Sánchez-Salcedo EM, Mena P, García-Viguera C, Hernández F, Martínez JJ. (Poly)phenolic compounds and antioxidant activity of white (Morus alba) and black (Morus nigra) mulberry leaves: Their potential for new products rich in phytochemicals. J. Funct. Foods. 2015;18:1039-1046.

26. Singh B, Makkar HPS. The potential of mulberry foliage as feed supplement in India. In: Mulberry for animal production. Anim. Health Prod. Paper. 2002;147:139-153.

27. Srivastava S, Kapoor R, Thathola A, Srivastava RP. Mulberry (Moms alba) leaves as human food: a new dimension of sericulture. Int. J. Food Sci. Nutrt. 2003;54(6):411-416.

28. Szőllősi R. Superoxide dismutase (SOD) and abiotic stress tolerance in plants: an overview. Ed.: Ahmad P. In: Oxidative damage to plants: Antioxidant networks and signaling. Academic press, 2014, 672 p.

29. Tausz M, Wonisch A, Grill D, Morales D, Jiménez MS. Measuring antioxidants in tree species in the natural environment: from sampling to data evaluation. J. Exp. Bot. 2003;54(387):1505-1510.

30. Thirumalaisamy R, Gowrishankar J, Sunganthapriya S, Prakash B, Ashok Kumar L, Arunachalam G. Genetic variability in Morus alba L. by biochemical and bioassay methods for increased silk productivity. J. Biomed. Sci. 2009;1:11-18.

31. Vijayan K, Saratchandra B, Teixeria da Silva JA. Germplasm conservation in mulberry (Morus spp.). Sci. Hortic. 2011a;128(4):371-379.

32. Vijayan K, Tikader A,da Silva JA. Application of tissue culture techniques for propagation and crop improvement in mulberry (Morus spp.). Tree Forest. Sci. Biotechnol. 2011b;5(1):1-13.

33. Yang NC, Jhou KY, Tseng CY. Antihypertensive effect of mulberry leaf aqueous extract containing γ-aminobutyric acid in spontaneously hypertensive rats. Food Chem. 2011;132:1796-1801.

34. Yao J, Yan B, Wang XQ, Liu JX. Nutritional evaluation of mulberry leaves as feed for ruminants. L. R. R. D. 2000.12(2).